午夜精品乱人伦小说区,高清国产av一区二区三区,99精品一区二区三区无码吞精,亚洲AV麻豆AⅤ无码电影

您好,歡迎進(jìn)入上海力敏實(shí)業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁(yè) >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 常規(guī)生化試劑 >> sigma現(xiàn)貨代理Dispase II D-4693

sigma現(xiàn)貨代理Dispase II D-4693

  • 更新時(shí)間:  2023-07-26
  • 產(chǎn)品型號(hào):  
  • 簡(jiǎn)單描述
  • sigma現(xiàn)貨代理Dispase II D-4693
    Related CategoriesApplication Index, Biochemicals and Reagents, Cell Biology, Dissociation, Enzymes, Inhibitors, and Substrates,
    More...
    activity ≥0.5 units/mg solid
    sol
詳細(xì)介紹

sigma現(xiàn)貨代理Dispase II D-4693

Description

Analysis Note

The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by divalent cations including Ca2+ , Mg2+, Mn2+ and Fe2+.

Preparation Note

A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Physical form

lyophilized powder containing calcium acetate and milk sugar

Application

The enzyme from Sigma has been used in developing a protocol for ex vivoculture of mouse embryonic mammary buds.[5] It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart.[6] It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.[1]
Dispase II has been used for Fluorescence-Activated Cell Sorting (FACS).[3] It has also been used for separating visceral yolk sac layers.[2]

Biochem/physiol Actions

Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.[4] Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity.[7] The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

sigma現(xiàn)貨代理Dispase II D-4693

我公司代理sigma公司試劑,大量現(xiàn)貨,和選購(gòu)


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
77777亚洲午夜久久多人| 性之剧毒在线观看| 亚洲一区二区三区影院| 超薄丝袜足j好爽在线| 丰满少妇高潮惨叫久久久| 国产精品无码久久av| 99国产精品久久久蜜芽| 亚洲欧美综合在线一区| 漂亮人妻被强了bd影片| 老牛精品亚洲成av人片| 7777久久亚洲中文字幕蜜桃| 老外又硬又粗又长又大| 黑人巨大精品欧美黑寡妇| 久久久精品妓女影院妓女网| 无码永久在线观看视频| chinese国产avvideoxxxx实拍 | 国产无遮挡裸体免费视频| 人人妻人人添人人爽欧美一区| 《色诱女教师》在线观看| 日本高清h色视频在线观看| 精品人妻无码一区二区三区淑枝| 人妻无码第一区二区三区| 成人av在线观看| 果冻传媒剧国产剧在线看 | 午夜福利视频| 亚洲最大中文字幕无码网站| 久久久久亚洲av无码a片| 国产999精品2卡3卡4卡 | 日本三级带黄在线观看| 香蕉久久夜色精品升级完成| 麻豆出品md004在线| 亚洲处破女a片出血| 国产又硬又粗进去好爽a片软件 | 日本精品videosse×少妇| 国产精品亚洲av| 人妻无码视频一区二区三区| 久久婷婷五月综合97色一本一本| 国产深夜男女无套内射| 香蕉久久夜色精品升级完成| 精品无码欧美一区二区三区不卡| 中文字幕无码亚洲字幕成a人蜜桃|